NIRUPAMA SINGH

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This case series describes two adult patients with B-cell acute lymphoblastic leukemia who had amplification of the MLL gene — a rare finding in this cancer type that carries a very poor prognosis. Both patients deteriorated rapidly and died within two to four months despite treatment, and a review of nine previously published cases with this genetic alteration confirmed consistently aggressive outcomes. The report reinforces the need for genetic profiling at diagnosis to identify this high-risk subgroup and develop better treatment strategies.

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This mixed-methods study in two Indian districts examined whether maternal and neonatal death review data could realistically be collected and used to identify gaps in care. Retrieving records was consistently difficult due to fragmented storage, unclear responsibility, and reluctance to share data, and most review forms lacked sufficient detail to assess whether deaths were preventable. Strengthening record-keeping and institutional data-sharing practices is essential before death reviews can fulfill their potential as a quality improvement tool.

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This study developed and tested two practical approaches for performing directional optical coherence tomography — an imaging technique that gives a cleaner measure of a specific retinal layer by changing the angle of the light beam. Both approaches (operator-adjusted beam angle and patient-adjusted fixation) produced consistent, reproducible outer nuclear layer thickness measurements comparable to manual expert analysis. The result is a clinically implementable method for a measurement that has been difficult to obtain outside research settings.

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This review examines how lipid molecules, non-coding RNAs, and interactions among retinal cell types contribute to abnormal blood vessel growth in the retina — a process underlying diabetic retinopathy, age-related macular degeneration, and retinopathy of prematurity. The authors argue for expanding research beyond the dominant VEGF signaling pathway to include these additional regulatory layers. Understanding how retinal cell types communicate to control vessel growth could open new therapeutic targets for blinding diseases.

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Researchers report the draft genome sequence of a multidrug-resistant bacterial strain — ASK-30 — isolated from wastewater in northern India. The genome contains multiple known antibiotic resistance genes. This genomic data provides a reference for tracking the spread of this resistance profile in environmental and clinical settings.

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This study tested whether a 405 nm blue LED device could kill wound pathogens on a synthetic collagen skin model that mimics human skin structure. The device achieved up to 3.5-log bacterial kill at 27 J/cm2, with broad activity against ESKAPE pathogens and Candida, and did not damage the collagen matrix even at high light doses. Blue light phototherapy emerges as a viable, contact-free, resistance-free option for wound infection management, with particular potential for reducing hospital-acquired infections.

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This paper proposes the VALID framework — a multi-layered quality assurance system — for evaluating clinical data extracted from medical records by large language models. The framework combines performance benchmarking against human experts, internal consistency checks, replication against established datasets, and demographic subgroup analysis to detect bias. By providing a structured, transparent validation process, VALID aims to establish standards for trustworthy AI-generated real-world evidence in oncology research.

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This single-center retrospective study followed 211 kidney transplant recipients with type 2 diabetes who were treated with GLP-1 receptor agonists over five years. Weight, HbA1c, and rates of major cardiovascular events all improved significantly, and kidney function remained stable or improved slightly — an unexpected finding in a population where kidney function typically declines. Larger prospective trials are needed, but this real-world experience suggests GLP-1 agents are safe and beneficial after kidney transplantation.

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This shotgun metagenomic study profiled antimicrobial resistance genes and bacterial communities in wastewater from 19 sites across four major Indian cities. Microbial community composition clustered by city, but antibiotic resistance gene patterns did not follow the same geographic structure. The study reveals that resistance genes spread across cities through shared mobile genetic elements, emphasizing the need for city-specific as well as national surveillance to track and contain antimicrobial resistance.

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This study developed a metal-free chemical method for synthesizing structurally diverse isoxazole compounds — a class of heterocycles used in pharmaceuticals — using a commercially available iodine-based reagent under mild conditions. The method works for multiple types of starting materials, tolerates a wide range of functional groups, and was demonstrated at gram scale, confirming practical utility. Photophysical studies showed that some of the new compounds have solvent-dependent fluorescence, suggesting potential applications in sensing or imaging.

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A patient who had lived with a lower extremity arteriovenous fistula — an abnormal connection between an artery and vein — for seven years after a machete injury presented with chronic venous insufficiency and nonhealing leg ulcers. Detailed imaging defined the complex anatomy, allowing targeted endovascular repair without open surgery. The case demonstrates that precise anatomic mapping enables minimally invasive treatment of even long-standing complex vascular injuries.

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This five-year study tracked antibiotic resistance in 988 Pseudomonas aeruginosa isolates from ICUs at a hospital in eastern India, finding that over half were resistant to key drugs like piperacillin-tazobactam, imipenem, and meropenem. Resistance to most drugs trended downward over the study period, and the highest drug susceptibility was to amikacin. The findings underscore the need for continuous antimicrobial stewardship in ICUs where critically ill patients are most vulnerable to drug-resistant infections.

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This network meta-analysis of 55 randomized trials covering 5,487 cesarean deliveries compared vasopressor infusions for preventing dangerous blood pressure drops under spinal anesthesia. Metaraminol, noradrenaline, phenylephrine, and adrenaline all outperformed no treatment, and agents with mild beta-adrenergic activity like noradrenaline and metaraminol best preserved fetal acid-base balance. Current guidelines recommending phenylephrine may need updating, as noradrenaline and metaraminol appear superior for both mother and baby.

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This study characterized the structure and antibacterial activity of micelles formed by mixing thyme essential oil with an imidazolium ionic liquid in water. The resulting micelles were about 400 nm in size, remained stable in water for at least six months, and achieved a 6-log reduction (complete kill) of both E. coli and Staphylococcus bacteria within one minute of exposure. These self-dispersing, water-stable antimicrobial micelles offer potential as natural alternatives to conventional disinfectants.

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This prospective multicenter study in India compared using heparinized saline versus contrast dye to flush coronary arteries during optical coherence tomography (OCT) imaging in 166 patients undergoing stenting procedures. All four measured vessel dimensions were statistically identical between saline and contrast flushes, with no safety events observed with saline. Saline is a safe, practical alternative to contrast for OCT-guided coronary intervention, which matters for patients at risk of kidney damage from contrast exposure.

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This study used molecular docking and antioxidant assays to test whether the natural compounds tyrosol and farnesol could interfere with key inflammatory targets in psoriasis. Both compounds bound effectively to psoriasis-associated proteins, showed significant free radical scavenging activity, and their combination had enhanced antioxidant effects compared to either alone. The results suggest tyrosol and farnesol together have potential as anti-psoriatic agents acting through anti-inflammatory and antioxidant mechanisms.

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This study developed a self-nanoemulsifying drug delivery system to improve oral absorption of lapatinib, a cancer drug with only 24% bioavailability. The new formulation kept the drug dissolved in a supersaturated state throughout digestion and achieved five times higher drug exposure in rats compared to the standard suspension. Strong correlations between lab permeability data and animal pharmacokinetics validate this approach as a rational strategy for improving oral delivery of poorly soluble cancer drugs.

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This review summarizes cardiovascular and kidney outcome data for oral GLP-1 receptor agonists — drugs that lower blood sugar and body weight — with a focus on their potential to prevent heart attacks and kidney disease in type 2 diabetes. Oral semaglutide reduced major cardiovascular events in the SOUL trial, but did not significantly reduce kidney outcomes, leaving injectable GLP-1 agents with the stronger evidence base for kidney protection. Newer oral agents like orforglipron show metabolic promise but lack cardiovascular and renal outcome data.

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A pregnant woman in her early 30s presented near term with a 10 cm mass growing from the labia minora, which was surgically removed following a cesarean section and confirmed to be a cellular angiofibroma — a rare benign tumor. The case highlights that these tumors can grow large enough during pregnancy to cause difficulty walking, and that surgical excision after delivery is safe and curative. Clinicians should include cellular angiofibroma in the differential diagnosis of vulvar masses in pregnant patients.

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This study assessed groundwater quality across 11 districts of southwestern Haryana, India using chemical analysis, quality indices, and spatial mapping. While most groundwater was suitable for irrigation, 15% of the area had drinking water quality classified as unsuitable, with chloride and sodium contamination posing noncarcinogenic health risks — especially for children. The study provides a district-level resource allocation framework and calls for real-time monitoring and tighter regulation of industrial and agricultural inputs.

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This case-control study in India tested 51 genetic variants outside the HLA region in 376 celiac disease patients and 736 healthy controls. Eighteen variants were significantly associated with celiac disease, with the strongest signals in genes involved in T cell receptor signaling, T cell activation, inflammatory cell movement, and intestinal barrier protection. The study maps the non-HLA genetic landscape of celiac disease in an Indian population and confirms T cell pathway genes as the dominant contributors beyond the known HLA risk.

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This proteomics study measured 276 proteins in cerebrospinal fluid and blood from 354 participants spanning the preclinical to prodromal stages of Alzheimer's disease. An elevated signature of vascular adhesion molecules — including ICAM-1, VCAM-1, and HGF — in the CSF was detectable even in people with no cognitive symptoms and predicted who would progress to more advanced disease. The findings point to early vascular and inflammatory dysfunction as a measurable feature of Alzheimer's pathology long before symptoms appear.

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This study tracked salivary levels of IGF-1 and bone alkaline phosphatase in 39 adolescents treated with a Twin Block appliance for jaw underdevelopment over the course of treatment. IGF-1 rose within the first week and BALP peaked at 6-10 weeks, with cephalometric analysis confirming significant increases in mandibular length in treated patients versus untreated non-compliant controls. The findings identify these two molecules as biochemical markers that reflect the bone-building response to functional jaw appliance therapy.

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This retrospective study compared 90-day complication rates and patient-reported outcomes in 23 patients with inflammatory arthritis versus 646 without it following minimally invasive lumbar fusion surgery. After matching for confounders, the two groups showed no significant differences in complications, reoperations, readmissions, or improvement in pain and function scores. Patients with inflammatory arthritis can expect outcomes from minimally invasive spinal fusion comparable to those without inflammatory disease.

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This systematic review examines how copper nanoparticles can be synthesized and used to kill cancer cells, covering their effects on breast, prostate, liver, lung, ovarian, colon, and cervical cancer cell lines. Copper nanoparticles cause cancer cell death through oxidative stress, DNA damage, and activation of apoptosis pathways, and can be targeted to tumor cells using surface coatings. The review positions copper nanoparticles as promising anticancer agents that can also be combined with radiation or chemotherapy for enhanced effect.

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This review covers the use of metal oxide and alkali salt nanoparticles as templates for making porous carbon materials with tailored structures for energy storage, environmental cleanup, and catalysis. Templates like MgO, ZnO, and NaCl are favored because they are cheap, thermally stable, and can be removed without harsh chemicals like hydrofluoric acid. The review provides a technical framework for researchers designing next-generation porous carbons for batteries, supercapacitors, and adsorption applications.

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Canadian stroke centers were surveyed about their practices for giving clot-dissolving drugs to stroke patients who had recently taken blood thinners (DOACs). Most centers had inconsistent access to DOAC level tests, and the majority did not routinely use those tests to guide treatment decisions because results took too long to return. The survey reveals major variability in practice and highlights the need for faster testing and clearer clinical guidelines.

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Researchers used a model of elevated eye pressure to map the 3D organization of gene regulation in the trabecular meshwork — the tissue that controls fluid drainage from the eye and is central to glaucoma pathology. By integrating this chromatin map with genetic risk data, they identified 26 candidate genes for elevated eye pressure and 52 for primary open-angle glaucoma, pointing to vesicle transport and several signaling pathways as key mechanisms. The study provides a detailed genetic blueprint that could guide development of new glaucoma treatments.

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Researchers tested whether two peptides derived from beef protein could block or reduce activity of the bitter taste receptor T2R14, which is found throughout the body and responds to over 100 bitter compounds. Both peptides significantly inhibited the receptor, and experiments showed that an enzyme called GRK2 is required for the receptor to turn off after activation. These findings could guide food scientists toward reducing bitterness in healthy but unpalatable foods and medications.

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This case report with 8-year follow-up describes successful dental implant placement in a patient with a narrow bone ridge, where part of the implant threads were exposed and required bone grafting and a barrier membrane at placement. At 8 years the bone had fully regenerated around the implant, with no inflammation and a well-functioning restoration confirmed by CBCT imaging. The case supports the effectiveness of guided bone regeneration at implant placement for patients with horizontal bone deficiency.

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This study compared standard pain management for chronic low back pain against a combined approach that added cognitive behavioral therapy (CBT). Patients receiving CBT in addition to standard care showed 25-29% improvement on a pain scale versus 18-20% for standard care alone, with similar advantages on disability and quality of life measures. Adding psychological counseling to routine physical treatments produces meaningfully better outcomes for chronic low back pain.