Manuel A Jusino

University of Florida, School of Forest, Fisheries, and Geomatics Sciences, Gainesville, FL, 32611, USA.

25 publications 2007 – 2025 ORCID

What does Manuel A Jusino research?

Manuel A Jusino studies fungi, specifically focusing on their biodiversity and cultivation methods. One area of his research involves cataloging diverse species of mushrooms and large fungi in Florida, where he discovered over 546 distinct species in a nine-year study. This is important because fungi play a vital role in forest ecosystems by helping trees obtain nutrients and decomposing organic material. Additionally, he researches how to cultivate valuable fungi like pecan truffles by experimenting with soil treatment techniques that enhance growth while fitting within existing agricultural practices.

Key findings

  • Over nine years, identified more than 546 fungal species at a single location in Florida, estimating a total of 900 to 1,200 species.
  • Found that treating the soil with chemicals to eliminate competing fungi combined with inoculating it with truffle spores resulted in the best growth outcomes for pecan tree seedlings.
  • Showed that transplanting pecan seedlings after 2-3 years significantly supports stronger truffle growth.

Frequently asked questions

Does Dr. Jusino study mushrooms?
Yes, Dr. Jusino studies a wide variety of mushrooms and fungi, focusing on their biodiversity and ecological roles.
What techniques has Dr. Jusino researched to grow truffles?
He has researched methods such as soil fumigation to eliminate competing fungi and inoculation with truffle spores to enhance growth of pecan truffles.
Is Dr. Jusino's work relevant to agriculture?
Yes, his work is relevant as it explores ways to cultivate valuable fungi, like pecan truffles, within existing agricultural frameworks.
What is the significance of fungi in ecosystems?
Fungi are crucial for ecosystems as they help trees absorb nutrients and decompose dead material, contributing to forest health and biodiversity.
How many species of fungi did Dr. Jusino discover at the Florida site?
He identified over 546 species and estimated that the total could be between 900 and 1,200 species at that site.

Publications in plain English

Invasive golden oyster mushrooms are disrupting native fungal communities as they spread throughout North America.

2025

Current biology : CB

Veerabahu A, Banik MT, Lindner DL, Pringle A, Jusino MA

Plain English
This study looked at how the invasive golden oyster mushroom is affecting local fungi in North America. Researchers found that where the golden oyster mushroom grows, there are fewer types of other fungi present; specifically, fungal diversity decreased significantly when it was present. In just eight years, this mushroom has spread to 25 states and one Canadian province, and climate change could allow it to invade even more areas. Who this helps: This information helps ecologists and environmental scientists who are working to protect local ecosystems.

PubMed

Salt Life: Salinity Drives Ectomycorrhizal Community Structure in the Endangered Pine Rocklands.

2025

Molecular ecology

Karlsen-Ayala E, Jusino MA, Smith ME, Gazis R

Plain English
This study looked at how rising salt levels in Florida's pine rocklands affect important fungi that help the endangered Pinus densa tree survive. Researchers found that as salinity increased, both the tree and the fungi faced more stress, leading to higher death rates of young trees. Specifically, seedlings exposed to higher salt concentrations showed more deaths and changes in the types of fungi present, which could hurt the tree's chances of thriving as sea levels rise. Who this helps: This information benefits conservationists and environmental scientists working to protect coastal ecosystems.

PubMed

Think globally, barcode locally: nine years of macrofungi sampling reveals extensive biodiversity at the ordway-swisher biological station, a subtropical site in Florida.

2025

Fungal biology

Caiafa MV, Kaminsky L, Healy R, Sheffer LP, Willis CB +26 more

Plain English
Researchers spent nine years cataloging mushrooms and other large fungi at a Florida nature preserve, identifying over 546 species and estimating the actual total is probably between 900 and 1,200 species—meaning there are more fungal species at this one site than there are vertebrate animals or plants. They used DNA testing to precisely identify each specimen and created a reference collection for future research. This matters because fungi are essential to forest ecosystems (they help trees absorb nutrients and break down dead material), yet scientists know far less about fungal diversity than they do about plants and animals, making this comprehensive catalog a crucial foundation for understanding and protecting Florida's ecosystems.

PubMed

Ectomycorrhizal fungal communities associated with Crocanthemum and Lechea (Cistaceae) in subtropical Florida sandhill habitats.

2024

Mycorrhiza

Caiafa MV, Grazziotti PH, Karlsen-Ayala E, Jusino MA, Healy R +3 more

Plain English
This study examined the types of fungi that live in partnership with two plants, Crocanthemum and Lechea, in sandy, fire-prone areas of Florida. Researchers found that these plants mostly hosted fungi from two groups: Cenococcum and Russula, with some fungi from the Delastria group also present. Although some fungi were shared with nearby pines, the fungal communities associated with Crocanthemum and Lechea were still notably different from those found with the pines. Who this helps: This research is beneficial to ecologists and conservationists studying plant-fungi relationships in unique ecosystems.

PubMed

Root-associated fungal communities are influenced more by soils than by plant-host root traits in a Chinese tropical forest.

2023

The New phytologist

Hogan JA, Jusino MA, Smith ME, Corrales A, Song X +5 more

Plain English
This study examined how the soil and the characteristics of tree roots influence the types of fungi that grow around tree roots in tropical forests in China. Researchers analyzed the roots of 150 trees across 66 species and found that soil phosphorus played a major role, accounting for 76% of the differences in fungal communities, while root features and chemistry explained a smaller portion. Understanding these relationships is important for forest health and biodiversity conservation. Who this helps: This benefits forest ecologists and conservationists.

PubMed

Preinvasion Assessment of Exotic Bark Beetle-Vectored Fungi to Detect Tree-Killing Pathogens.

2022

Phytopathology

Li Y, Bateman C, Skelton J, Wang B, Black A +20 more

Plain English
This study examined fungi carried by certain beetles from Asia and Europe to see if they pose a threat to trees in North America. Researchers tested 111 fungi and found that while 22 were identified as minor pathogens, none were highly dangerous, easing fears about these potential invaders. This matters because it helps forestry officials focus their efforts on managing risks before harmful species arrive, ensuring better protection for trees and forests. Who this helps: This helps forest managers and policymakers in protecting North American trees.

PubMed

Understudied, underrepresented, and unknown: Methodological biases that limit detection of early diverging fungi from environmental samples.

2022

Molecular ecology resources

Reynolds NK, Jusino MA, Stajich JE, Smith ME

Plain English
This study looked at methods used to identify early diverging fungi (EDF) in environmental samples, focusing specifically on a group called Zoopagomycota. The researchers tested different DNA markers and found that their choice significantly affects which fungi are detected; for example, using standard methods missed a lot of EDF, leading to incomplete pictures of fungal diversity. Improving detection techniques is crucial because understanding these lesser-known fungi can provide insights into ecosystem health and biodiversity. Who this helps: This helps researchers and environmental scientists studying fungal diversity and ecosystem dynamics.

PubMed

Disease-related population declines in bats demonstrate non-exchangeability in generalist predators.

2022

Ecology and evolution

Wray AK, Gratton C, Jusino MA, Wang JJ, Kochanski JM +4 more

Plain English
Researchers studied how declining bat populations due to a disease called white-nose syndrome affect the diets and roles of surviving bat species in North America. They found that after little brown bat populations sharply declined, both little brown and big brown bats ate less diverse prey, particularly agricultural pests. This shows that remaining bats don’t fill the gaps left by species that are diminishing, which is important for understanding how ecosystems function and the roles different species play. Who this helps: This information benefits ecologists and conservationists working to protect bat populations and manage ecosystems.

PubMed

Effects of cavity orientation on nesting success inferred from long-term monitoring of the endangered red-cockaded woodpecker.

2022

Scientific reports

Landler L, Skelton J, Jusino MA, Van Lanen A, Walters JR

Plain English
This study looked at how the direction red-cockaded woodpeckers build their nesting cavities affects their success in raising chicks. Researchers found that these woodpeckers tended to create their nests with entrances facing west, which helped improve the number of eggs that hatched and the number of baby woodpeckers that survived. Specifically, they discovered that better orientation of the cavities was linked to higher hatching and fledging rates, making these findings crucial for understanding how to conserve this endangered species effectively. Who this helps: This helps conservationists and wildlife managers who work to protect red-cockaded woodpeckers.

PubMed

Predator preferences shape the diets of arthropodivorous bats more than quantitative local prey abundance.

2021

Molecular ecology

Wray AK, Peery MZ, Jusino MA, Kochanski JM, Banik MT +3 more

Plain English
This study looked at how two types of bats, the little brown bat and the big brown bat, choose their food, focusing on how their preferences for certain insects matter more than how many of those insects are available. Researchers found that even when the number of insects changed a lot depending on the season and location, the bats consistently preferred certain types of insects, regardless of how common they were. This shows that bats have specific food choices that influence their diets, highlighting the complexity of food webs in nature. Who this helps: This benefits researchers studying bat behavior and conservation efforts aimed at protecting these bat species.

PubMed

Discovering the role of Patagonian birds in the dispersal of truffles and other mycorrhizal fungi.

2021

Current biology : CB

Caiafa MV, Jusino MA, Wilkie AC, Díaz IA, Sieving KE +1 more

Plain English
This study looked at how certain birds in Patagonia help spread truffles and other fungi by eating them and then passing the spores in their droppings. Researchers found that two common bird species, the chucao tapaculo and the black-throated huet-huet, regularly eat these fungi and effectively disperse their spores, which benefits the trees and plants in their environment. Specifically, the birds consumed a variety of mycorrhizal fungi, supporting the growth of around 30 different types, which help trees in the forest get nutrients. Who this helps: This research helps environmental scientists and conservationists understand the important role of birds in forest ecosystems.

PubMed

Effects of Field Fumigation and Inoculation With the Pecan Truffle () on the Fungal Community of Pecan () Seedlings Over 5 Years.

2021

Frontiers in microbiology

Grupe AC, Jusino MA, Mujic AB, Spakes-Richter B, Bonito G +2 more

Plain English
Researchers spent five years testing whether they could grow pecan truffles (expensive, flavorful fungi) on pecan tree seedlings by treating soil with chemicals to kill competing fungi and then adding truffle spores. They found that treating the soil and adding truffles together worked best, and that seedlings should be transplanted after 2-3 years for the strongest truffle growth. The results show that pecan truffles can be grown using methods that fit into existing pecan nursery operations, opening the door to commercially farming these valuable fungi alongside pecan trees.

PubMed

A total crapshoot? Evaluating bioinformatic decisions in animal diet metabarcoding analyses.

2020

Ecology and evolution

O'Rourke DR, Bokulich NA, Jusino MA, MacManes MD, Foster JT

Plain English
This study looked at how different computer methods used in analyzing animal diets can affect the results of biodiversity assessments. They found that using advanced denoising techniques led to fewer errors than traditional methods and highlighted that different databases for classifying animal genes can give very different results—sometimes assigning many fewer species names than other methods. This matters because inconsistent practices can lead to inaccurate conclusions about animal diets, making it hard to compare research across studies. Who this helps: This helps researchers and conservationists working on animal diet studies.

PubMed

Major histocompatibility complex variation is similar in little brown bats before and after white-nose syndrome outbreak.

2020

Ecology and evolution

Yi X, Donner DM, Marquardt PE, Palmer JM, Jusino MA +3 more

Plain English
This study looked at the genetic variation in little brown bats before and after the white-nose syndrome outbreak, which has hurt bat populations across North America. Researchers examined 131 bats and found that the genes related to their immune system did not show significant changes due to the disease, suggesting that other factors, rather than genetic adaptation, are helping bats survive in these impacted areas. Specifically, while the number of different genetic traits decreased after the outbreak, the overall immune gene variation remained the same. Who this helps: This research benefits conservationists and wildlife biologists working to protect bat populations.

PubMed

An improved method for utilizing high-throughput amplicon sequencing to determine the diets of insectivorous animals.

2019

Molecular ecology resources

Jusino MA, Banik MT, Palmer JM, Wray AK, Xiao L +6 more

Plain English
This study focused on improving how scientists analyze the diets of insect-eating animals, like bats, by using DNA from their droppings. Researchers found that a new method using specific DNA markers (the ANML primer) successfully identified 98% of different insect types compared to just 41-68% with an older method (the ZBJ primer). This is important because it helps to get a clearer picture of the diets of these animals and can better track important insects like mosquitoes that affect health and economies. Who this helps: This helps researchers studying wildlife, pest control specialists, and public health officials.

PubMed

A selective fungal transport organ (mycangium) maintains coarse phylogenetic congruence between fungus-farming ambrosia beetles and their symbionts.

2019

Proceedings. Biological sciences

Skelton J, Johnson AJ, Jusino MA, Bateman CC, Li Y +1 more

Plain English
This study looked at how certain beetles, known as ambrosia beetles, partner with specific types of fungi to farm them. Researchers found that these beetles have a special organ that helps them stick with certain fungal lineages while still allowing some flexibility with closely related fungi. They discovered that this organ is essential for maintaining relationships with their fungal partners, as beetles that lost this organ switched to different fungi more often. Who this helps: This helps researchers and ecologists understand the relationships between insects and fungi, which is important for forest health and biodiversity.

PubMed

Relationships among wood-boring beetles, fungi, and the decomposition of forest biomass.

2019

Molecular ecology

Skelton J, Jusino MA, Carlson PS, Smith K, Banik MT +3 more

Plain English
This study examined how wood-boring beetles affect the fungi that help decompose pine trees in forests. Researchers found that when beetles were allowed access to the trees, they increased the number of different fungal types living there but did not significantly promote wood decay—actually, they seemed to slow it down by competing with fungi that usually break down wood. This challenges the common belief that beetles mostly help with wood decomposition and highlights the complex role they play in forest ecosystems. Who this helps: This research benefits ecologists and forest managers.

PubMed

Detecting Symbioses in Complex Communities: the Fungal Symbionts of Bark and Ambrosia Beetles Within Asian Pines.

2018

Microbial ecology

Skelton J, Jusino MA, Li Y, Bateman C, Thai PH +3 more

Plain English
This study looked at the fungi that live with bark and ambrosia beetles found in Asian pines in China and Vietnam. Researchers found that almost half of the differences in fungal types depended on the beetle species involved. Some fungi only had a close relationship with one type of beetle, while others were shared among various beetles, indicating that the relationships between these fungi and beetles can vary widely. Who this helps: This research benefits scientists studying ecological relationships and forest health.

PubMed

Non-biological synthetic spike-in controls and the AMPtk software pipeline improve mycobiome data.

2018

PeerJ

Palmer JM, Jusino MA, Banik MT, Lindner DL

Plain English
This study looked at ways to improve the analysis of fungal DNA sequences in microbial communities. Researchers created two types of test samples—one based on real fungi and one made from synthetic DNA—finding that the synthetic samples provided the best accuracy for data analysis processes. They concluded that proper techniques, especially during the initial DNA copying stage, are crucial for getting reliable results, and they developed a new software tool (AMPtk) to help analyze this data more effectively. Who this helps: This benefits researchers and scientists studying fungi in various environments, helping them obtain clearer and more accurate data.

PubMed

Identifying the "Mushroom of Immortality": Assessing theSpecies Composition in Commercial Reishi Products.

2018

Frontiers in microbiology

Loyd AL, Richter BS, Jusino MA, Truong C, Smith ME +2 more

Plain English
This study looked at different types of reishi mushrooms sold in various products, such as pills and teas. Researchers found that 93% of commercial reishi products contained a type of mushroom common in Asia, but none had the specific type known for its health benefits. The study highlights that many products don't match their labels, which is important for consumers to know since they might be using them for medicinal purposes. Who this helps: This benefits consumers and patients who use reishi products for health reasons.

PubMed

Wood decay fungus Flavodon ambrosius (Basidiomycota: Polyporales) is widely farmed by two genera of ambrosia beetles.

2017

Fungal biology

Li Y, Bateman CC, Skelton J, Jusino MA, Nolen ZJ +2 more

Plain English
Researchers studied a fungus called Flavodon ambrosius, which is a key food source for certain beetles known as ambrosia beetles in North America. They found that this fungus is not only present in North America but also in Asia, where it forms a partnership with several species of beetles. This fungus and beetle relationship is important because it is the most widespread mutualistic fungus known, benefiting multiple beetle species across the globe. Who this helps: This research helps scientists and farmers understand beetle-fungus relationships, which can inform pest management strategies.

PubMed

Management of an Immediate Implant Horizontal Defect Using Freeze-Dried Bone Allograft and a Neodymium:Yttrium-Aluminum-Garnet Laser.

2017

Clinical advances in periodontics

Johnson TM, Jusino MA

Plain English
This study looked at a new method for fixing bone defects around dental implants using freeze-dried bone and a special laser. A patient had a tooth removed and received an implant, with the laser helping to keep the area stable. After 31 months, the bone levels looked good and the area healed well, showing that this method can be effective when temporary dental covers aren't possible. Who this helps: This helps dentists and their patients needing immediate implants.

PubMed

Amplicon-Based Sequencing of Soil Fungi from Wood Preservative Test Sites.

2017

Frontiers in microbiology

Kirker GT, Bishell AB, Jusino MA, Palmer JM, Hickey WJ +1 more

Plain English
This study looked at soil samples from two different wood treatment sites in North America, aiming to see how long-term use of wood preservatives affects soil fungi. The researchers found that the types of fungi varied significantly between the two sites, and after 50 years of exposure, different preservatives influenced fungal communities in different ways. Importantly, while some harmful chemicals were detected, they were at levels similar to those found in unexposed areas, suggesting that the preservatives might not be excessively harmful. Who this helps: This information benefits environmental scientists and land managers focused on soil health.

PubMed

Experimental evidence of a symbiosis between red-cockaded woodpeckers and fungi.

2016

Proceedings. Biological sciences

Jusino MA, Lindner DL, Banik MT, Rose KR, Walters JR

Plain English
This study looked at the relationship between red-cockaded woodpeckers and fungi to see how they help each other. Researchers found that these woodpeckers not only help spread fungi but also that the fungi assist the woodpeckers by softening wood for easier excavation. After examining various tree holes, they discovered that the areas the woodpeckers used had similar fungal communities, while areas they couldn't access were very different, highlighting their strong partnership. Who this helps: This benefits forest conservationists and wildlife managers.

PubMed

Structural basis for ligand and heparin binding to neuropilin B domains.

2007

Proceedings of the National Academy of Sciences of the United States of America

Vander Kooi CW, Jusino MA, Perman B, Neau DB, Bellamy HD +1 more

Plain English
This study looked at how a receptor called neuropilin (Nrp) interacts with two important proteins, VEGF and semaphorin, which are involved in blood vessel formation and nerve growth. Researchers discovered the structure of parts of the Nrp receptor and found that a small molecule, heparin, helps these parts work together better. Understanding these interactions is crucial because it can help improve therapies for diseases where nerve and blood vessel growth goes wrong. Who this helps: This research benefits patients with conditions related to abnormal blood vessel and nerve development, as well as doctors aiming to treat these issues.

PubMed

Publication data sourced from PubMed . Plain-English summaries generated by AI. Not medical advice.